Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life.
— Randy Schekman
Although synapses differ in properties, all synapses operate by the same principle.
— Sir Bernard Katz
Progress in science depends on new techniques, new discoveries and new ideas, probably in that order.
— Sydney Brenner
Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life.
— Randy Schekman
What I cannot create, I do not understand.
— Richard P. Feynman
Atoms are not indivisible, for negatively electrified particles can be torn from them by the action of electrical forces.
— Sir J.J. Thomson
My own prejudices are exactly the opposite of the functionalists’: “If you want to understand function, study structure”.
— Francis Crick
Tissue clearing methods Introduction This webinar will explain tissue clearing, how it functions, and the three main methods for making tissues transparent. Moreover, the advantages and limitations of various tissue cleansing methods.
Organic solvent-based tissue clearing PEGASOS (Using in our lab, recommended): PEGASOS version 1：Polyethylene glycol(PEG) Associated Solvent System
Jing, Dian, et al. “Tissue clearing of both hard and soft tissue organs with the PEGASOS method.” Cell research 28.8 (2018): 803-818.
RNA-Seq KITS FOR RNA EXTRACTION Takara: Smart-Seq
SEQUENCING PLATFORM illumina; Huada Gene
ANALYSIS SOFTWARES QC:
fastqc: FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis.
Believing is seeing and seeing is believing.
— Tom Hanks
R R-The R Project for Statistical Computing: https://www.r-project.org/
Rstudio: Rstudio Workbench is an integrated development environment for R and Python, with a console, syntax-highlighting editor that supports direct code execution, and tools for plotting, history, debugging and workspace management.
RShiny: Shiny is an R package that makes it easy to build interactive web apps straight from R. You can host standalone apps on a webpage or embed them in R Markdown documents or build dashboards.
