Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life.
— Randy Schekman
Although synapses differ in properties, all synapses operate by the same principle.
— Bernard Katz
Progress in science depends on new techniques, new discoveries and new ideas, probably in that order.
— Sydney Brenner
Our cells engage in protein production, and many of those proteins are enzymes responsible for the chemistry of life.
— Randy Schekman
What I cannot create, I do not understand.
— Richard P. Feynman
Atoms are not indivisible, for negatively electrified particles can be torn from them by the action of electrical forces.
— Sir J.J. Thomson
CroyEM system | Sample preparation 1.
Methods to improve particle behavior
Size exclusion chromatography: Gel filtration can be a powerful tool to predict structural heterogeneity. Whenever possible, gel filtration should be incorporated to the final steps of a purification protocol. Protein or protein complex stabilization: If negative stain or cryo-EM analysis reveals significant structural heterogeneity, then the user may consider additional methods to stabilize a structural target in a defined conformation.
Introduction This webinar will explain tissue clearing, how it functions, and the three main methods for making tissues transparent. Moreover, the advantages and limitations of various tissue cleansing methods.
PEGASOS (Using in our lab, recommended): PEGASOS version 1：Polyethylene glycol(PEG) Associated Solvent System
Jing, Dian, et al. “Tissue clearing of both hard and soft tissue organs with the PEGASOS method.” Cell research 28.8 (2018): 803-818. [PDF | DOI Link] TESOS system:
RNA-Seq KITS FOR RNA EXTRACTION Takara: Smart-Seq
SEQUENCING PLATFORM illumina; Huada Gene
ANALYSIS SOFTWARES QC:
fastqc: FastQC aims to provide a simple way to do some quality control checks on raw sequence data coming from high throughput sequencing pipelines. It provides a modular set of analyses which you can use to give a quick impression of whether your data has any problems of which you should be aware before doing any further analysis.
Believing is seeing and seeing is believing.
— Tom Hanks
R R-The R Project for Statistical Computing: https://www.r-project.org/
Rstudio: Rstudio Workbench is an integrated development environment for R and Python, with a console, syntax-highlighting editor that supports direct code execution, and tools for plotting, history, debugging and workspace management.
RShiny: Shiny is an R package that makes it easy to build interactive web apps straight from R. You can host standalone apps on a webpage or embed them in R Markdown documents or build dashboards.
