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Hello, My name is Jun-Liszt Li. Currently, I am a graduate student in PKU-CIBR joint program of Peking University. My long-term goal is to discover and characterize basic mechanisms underlying brain aging and neuron growth. In addition, I working on Next-generation omics (scRNAseq; Spatial metabolomics) technology, next-generation microscopy (Lightsheet, Confocal, 2p), and next-generation gene therapy (AAV, promoter) in order to apply these technologies to address specific problems.

Interest Areas

  • Biological Question
    Brain vasculature system
    Brain metabolic system

  • Technology
    scRNAseq & Spatial transcriptome
    Spatial metabolomics
    AAV engineering
    Promoter engineering

Education

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Ph.D., Peking University, Beijing
SEPTEMBER 2018 — January 2024
Academy for Advanced Interdisciplinary Studies (AAIS)
Major in Neurobiology; CIBR-PKU-joint Ph.D. program

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Bachelor, Huazhong Agricultural University, Wuhan
SEPTEMBER 2014 — JUNE 2018
College of Life Science and Technology
Major In Bioscience; Class of State Key Educational Base

This is my CV, click and view.

Research Experiences

Graduate student, Peking University & Chinese institute for Brain Research (CIBR)
MARCH 2019 — DECEMBER 2023
Graduate student advised by Dr.Wooping Ge, Ph.D. and Dr.Wenzhi Sun, Ph.D.

  • Focusing on AAV capsid engineering and make molecular/genetic tools for study of brain vasculature system.
  • Applying Next-generation microscopy (Confocal, Lightsheet, Two photon) technology into study of brain vasculature system.
  • Applying Next-generation omics technology (Transcriptomics; Metabolomics) to study the molecular architecture of brain metabolic system.

Rotation student, National institute of Biological Science (NIBS)
DECEMBER 2018 — FEBRUARY 2019
Rotation student advised by Dr.Qinghua Liu, Ph.D.

  • Learning knowledge and research approaches(Genetic screen) in the Sleep field.
  • Designing and implementing Automatic sleep staging system based on manifold and deep learning algorithm(EEG/EMG data processing); Automatic video tracking based sleep staging system based on computer vision algorithms.

Rotation student, Institute of Biophysics (IBP), Chinese Academy of Sciences
SEPTEMBER 2018 — DECEMBER 2018
Rotation student advised by Dr.Xiaoqun Wang, Ph.D.

  • Learning single cell sequencing technology including Patch-seq, Drop-seq.
    Skills including: single cell isolation, library construction, scRNA-seq data analysis( Linux system operation, R statistic analysis).

Undergraduate Researcher , National institute of Biological Science (NIBS)
JANUARY 2018 — SEPTEMBER 2018
Undergraduate Researcher advised by Dr.Minmin Luo, Ph.D

  • Designing and implementing a cell lethality Reporter(CLR) based system for orphan GPCRs high throughput screening(HTS) and novel kinase activity Reporter system

Undergraduate Researcher, HuaZhong Agricultural University
SEPTEMBER 2015 — JANUARY 2017
Undergraduate Researcher advised by Dr.Gang Cao, Ph.D.

  • Verification of MagR assembly and probing the mechanism of cellular calcium homeostasis
  • Optimize the MagR-based magneto-genetics tool and develop MagR2.0 for non-invasive manipulation of neuronal activity.
  • MTB(Mycobacterium tuberculosis) gene overexpression and knockout(CRISPR system)

HZAU-iGEM team member, Inovation Lab, College of Life science and technology, HZAU
FEBRUARY 2015 - OCTOBER 2016
2016 HZAU-iGEM team member with instructor Dr.Bin-Guang Ma, PhD

  • Systems and Synthetic Biology learning: student presentation, case-based learning, and discussion
  • During the Competition and in our project, I am responsible for the Optical control part which uses light switchable circuit (LSC) to control the expression of bacteria movement related gene CheZ.
  • Website of HZAU-iGEM2016: http://2016.igem.org/Team:HZAU-China

2015 HZAU-iGEM team member with instructor Dr.BingGuang Ma, PhD

  • Systems and Synthetic Biology learning: student presentation, case-based learning, and discussion
  • During the Competition and in our project, I am responsible for the interface part. We choose the light and use Optogenetics technique to regulate the synthetic gene oscillator in bacteria.
  • Website of HZAU-iGEM2015: http://2015.igem.org/Team:HZAU-China

Posters & Conferences:

SEPTEMBER 2015 — PRESENT

  1. Attendee, High-end-Symposium of Metabolomics and exposomics, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, 2021, Dalian, China
  2. Online Attendee, Beijing International Conference on Brain Science, 2020, Beijing, China
  3. Conference Volunteers, Beijing “Brain and Behavior” International Symposium and Zhongguancun Life Science Park Development Symposium, 2019, Beijing, China
  4. Attendee, Beijing International Academic Exchange Season-Brain Science and Brain-like Intelligence Research Symposium, Beijing Normal University, 2018, Beijing, China
  5. Attendee, International Workshop of Neuron Reconstruction and Applications, 2018, SEU-Allen Joint Institute, Nanjing, China
  6. P.C.,H.K.J.,B.Y.Z.,J.L.,Z.X.D.,X.X.,B.C.C.Q.L.Z.,B.G.M.et.al. Bio-Pattern Formation in Augmented Reality (BioPaFiAR), Gaint Jambree iGEM meeting, 2016, MIT Cambridge, Massachusetts, USA
  7. L.Q.D.,L.Z.Y.,Y.K.J.,Z.’.,Z.’.Y.J.’.,L.J.- ,C.P.,X.Y.B.,H.K.J.,X.S.’.,L.S.J.,G.Q.Q.,Q.Y.,B.G.M.,Mixed-Reality Cell-Bidirectional coupling between real and virtual bio-oscillators, Gaint Jambree iGEM meeting, 2015, MIT Cambridge, Massachusetts, USA
  8. L.Q.D.,L.Z.Y.,Y.K.J.,Z.’.,Z.’.Y.J.’.,L.J.,- C.P.,X.Y.B.,H.K.J.,X.S.’.,L.S.J.,G.Q.Q.,Q.Y.,B.G.M.,Mixed-Reality Cell-Bidirectional coupling between real and virtual bio-oscillators, CCIC(Conference of China iGEMers Committee)meeting, 2015, Peking University, Beijing, China

Honors & Awards

APRIL 2014 — PRESENT

Honors

  1. China Optics Valley Scholarship, 12/2022, Optical Valley Foundation, China
  2. Excellent student Scholarship of Baosteel, 10/2021, (Baosteel Education Foundation), China
  3. Outstanding Graduates, 06/2018, College of life Science and Technology, HZAU
  4. Dean Scholarship (The highest scholarship that represents student’s academic performance), 09/2017, College of life Science and Technology, HZAU
  5. National Endeavor Scholarship, 09/2017, Hubei Province Department of Education, China
  6. Qifa Zhang Scholarship (Funded by Qifa Zhang who is an academician of Chinese academy of sciences, foreign academician of the American academy of sciences and The third world academy of sciences), 10/2015, College of life Science and Technology, HZAU
  7. Freshman Principle special Scholarship (Scholarship for Freshman year student honored by the president of HZAU), 09/2014, Huazhong Agricultural University
  8. Model Student of Academic Records, 10/2016, College of life Science and Technology, HZAU
  9. Triple-A Student, 2017, College of Life Science and Technology, HZAU
  10. Triple-A Student, 2015, College of Life Science and Technology, HZAU

Awards

  1. Bronze medal of iGEM-2016 Competition (International Genetically Engineered Machine Competition), 11/2016, MIT Cambridge, Massachusetts, USA
  2. Gold medal and Best nomination of iGEM-2015 Competition (International Genetically Engineered Machine Competition), 09/2015, MIT Cambridge- , Massachusetts, USA
  3. First prize of Academic excellence,03/2017, College of Life science and technology, HZAU
  4. First prize of Academic excellence,04/2015, College of Life science and technology, HZAU
  5. Second prize of Molecular biology Experiment Skill and Operation Contest, 06/2017, Office of Educational Administration, HZAU
  6. Second prize of Genetics Experiment Skill and Operation Contest, 06/2016, Office of Educational Administration, HZAU
  7. Second prize of Plant Biology Experiment Skill and Operation Contest, 07/2015, Office of Educational Administration, HZAU